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INCENP [G9-D2] Catalog Number：OM627784

點(diǎn)擊次數(shù)：29 發(fā)布時(shí)間：2024/9/10 11:13:07
 

Product Profile

Product Name INCENP [G9-D2]
Antibody Type Primary Antibodies
Product description 
A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H ａnd INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle ａnd are involved in the formation of the centromere ａnd the organization ａnd function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules ａnd interacts with AURKB, AURKC, CBX3 ａnd β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes ａnd during anaphase, to the equatorial cortex.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBICCIHCFC
 
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:100-1:200
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Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
binds ａnd activates aurora B ａnd C in vivo ａnd in vitro antibody Chromosomal passenger protein antibody INCE_HUMAN antibody INCENP antibody Inner centromere protein antibody Inner centromere protein antigens 135/155kDa antibody Inner centromere protein antigens 135kD 155kD antibody Inner centromere protein INCENP antibody
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Molecular Weight(MW) 105 kDa
Cellular Localization Cytoskeleton. Nucleus.

Database Links

SwissProt ID Q9NQS7

Application

  • Application

    Fig1: Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of INCENP on HEK293 (1) ａnd INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution.

  • Application

    Fig3: ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4:Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human INCENP recombinant protein, INCENP-hIgGFc transfected HEK293 cell lysate, human cervical cancer tissue, human rectum cancer tissue, HepG2, Jurkat.
Application Notes
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:100-1:200
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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