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聯(lián)系我們
聯(lián)系人：楊燕燕
技術文章
Anti-INCENP antibody Catalog Number：OM642642
點擊次數(shù)：23 發(fā)布時間：2024/9/10 11:11:22
Product Profile
| Product Name | Anti-INCENP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
Target Information
| Gene Symbol | INCENP |
|---|---|
| Gene Full Name | inner centromere protein |
| Gene Summary | In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins ａnd ＇passenger,＇ or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), ａnd CENPD (MIM 117142). The term ＇passenger proteins＇ encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw ａnd Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cylasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); ａnd CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw ａnd Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008] More |
| Molecular Weight(MW) | 105KD |
| Cellular Localization | Centromere, Chromosome, Cylasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus |
Application
-
WB
Western blot analysis using INCENP antibody against K562(1) cell lysate.
-
IHC
Immunohistochemical analysis of paraffin-em
bedded liver cancer tissues using INCENP antibody with DAB staining. -
ICC/IF
Immunofluorescence analysis of Hela cells using INCENP antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
| Application Notes | WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500 |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
原創(chuàng)作者：上海篤瑪生物科技有限公司
