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技術(shù)文章

SPIB Antibody Catalog Number：OM292064

點擊次數(shù)：34 發(fā)布時間：2024/9/9 14:20:08
 

Product Profile

Product Name SPIB Antibody
Antibody Type Primary Antibodies
Product description 
SPI1 (MIM 165170) ａnd SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB ａnd SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).1) Geng, C.D. (2005) J. Biol. Chem. 280 (52), 43264-43271.
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Immunogen 
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPIB.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAWB
 
SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

DogHumanMouseRatZebra Fish
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SPIB
Alternative Names
SPIB
SPI-B
Molecular Weight(MW) 19 kDa

Database Links

Entrez Gene 6689
Protein Accession NP_003112

Application

  • Application

    Antibody used in WB on Human Daudi at 0.2-1 ug/ml.

Positive Control 1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPIB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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