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技術文章

SPIB Antibody Catalog Number：OM292065

點擊次數(shù)：27 發(fā)布時間：2024/9/9 14:19:08
 

Product Profile

Product Name SPIB Antibody
Antibody Type Primary Antibodies
Product description 
SPIB is a member of the ETS transcription factor family that influences lymphoid development ａnd activity ａnd binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hemaoietic cells such as B cells ａnd plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development ａnd antigenic stimulation (3,4).1) Ray D, Bosselut R, Ghysdael J, et al. Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1. Mol. Cell Biol. 1992; 12:4297-304.
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Immunogen 
SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
 
SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SPIB
Alternative Names
Spi-B transcription factor
SPI-B
Molecular Weight(MW) Predicted: 19, 25, 29 kDa Observed: 20 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Pig: (88%)
Tissue Specificity SPIB antibody is human, mouse ａnd rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

Database Links

Entrez Gene 6689
Protein Accession NP_003112

Application

  • Application

    Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/mL.

  • Application

    Immunohistochemistry of SPIB in human prostate tissue with SPIB antibody at 2.5 μg/ml.

  • Application

    Western blot analysis of SPIB in human prostate tissue lysate with SPIB antibody at 1 μg/ml.

Positive Control 1) Cat. No. 1312 - Human Prostate Tissue Lysate
Application Notes
SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.:

Additional Information

Form Liquid
Storage Instructions SPIB antibody can be stored at 4˚C for three months ａnd -20˚C, stable for up to one year.
Storage Buffer SPIB antibody is supplied in PBS containing 0.02% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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