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技術(shù)文章

SPIB Catalog Number：OM630867

點(diǎn)擊次數(shù)：32 發(fā)布時(shí)間：2024/9/9 14:17:49
 

Product Profile

Product Name SPIB
Antibody Type Primary Antibodies
Product description 
Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5＇-GAGGAA-3＇) that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon ａnd block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development ａnd antigenic stimulation.
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Immunogen 
KLH conjugated synthetic peptide derived from human SPIB 181-262/262

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
 
WB:1:500-2000:
Species Reactivity

HumanMouseRatCowHorseRabbitSheep
Concentration 1 mg/mL.

Target Information

Alternative Names
SPI B Spi B transcription factor Spib SPIB_HUMAN Transcription factor Spi-B.
Molecular Weight(MW) 29 KDa
Cellular Localization Cylasm ａnd Nucleus.

Database Links

SwissProt ID
Q01892

Application

  • Application

    Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD

  • Application

    Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-SPIB (ER1917-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD

Positive Control Expressed in plasmacytoid dendritic cells (pDCs) ａnd B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
Application Notes
WB:1:500-2000:

Additional Information

Form Liquid
Storage Instructions Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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