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技術(shù)文章

SPIB Catalog Number:OM640801

點(diǎn)擊次數(shù):29 發(fā)布時(shí)間:2024/9/9 14:16:46
 

Product Profile

Product Name SPIB
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCICCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

HumanMouse

Target Information

Alternative Names
SPI-B
Molecular Weight(MW) 28.8kDa

Database Links

Entrez Gene 6689

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)

  • Application

    Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.

  • Application

    Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Application

    Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).

  • Application

    Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.

  • Application

    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者:上海篤瑪生物科技有限公司

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