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技術(shù)文章
Rabbit anti-ANXA1 polyclonal antibody - N-terminal region Catalog Number：OM107879
點(diǎn)擊次數(shù)：23 發(fā)布時(shí)間：2024/9/9 13:20:00
Product Profile
| Product Name | Rabbit anti-ANXA1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-ANXA1 antibody: synthetic peptide directed towards the N terminal of human ANXA1 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | BovineDogGuinea PigHumanMousePig |
| Concentration | 1 mg/ml |
| Purification | Protein A |
Target Information
| Gene Symbol | ANXA1 |
|---|---|
| Gene Synonyms | |
| Gene Full Name | Annexin A1 |
| Gene Summary | ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity. More |
| Alternative Names | |
| Molecular Weight(MW) | 38kDa |
| Sequence | 346 amino acids |
Database Links
| Entrez Gene | 301 |
|---|---|
| SwissProt ID | Q5TZZ9 |
| Protein Accession | NP_000691 |
Application
-
Immunohistochemical
-
Western blot
Host: Rabbit
Target Name:ANXA1
Sample Tissue:HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration:2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12% -
Western blot
Positive Control: Lane 1: 20ug mouse fibroblast lysates
Primary Antibody Dilution : 1:600
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Anonymous -
Western blot
1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot ａnd store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
原創(chuàng)作者：上海篤瑪生物科技有限公司
