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技術(shù)文章

ANXA1 Antibody Catalog Number：OM271186

點擊次數(shù)：42 發(fā)布時間：2024/9/6 14:39:31
 

Product Profile

Product Name ANXA1 Antibody
Antibody Type Primary Antibodies
Product description 
ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 ａnd are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins ａnd leukotrienes, annexin I may have potential anti-inflammatory activity.1) Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676.
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Immunogen 
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAIHCWB
 
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol ANXA1
Alternative Names
ANXA1
ANX1
LPC1
Molecular Weight(MW) 38 kDa

Database Links

Entrez Gene 301
Protein Accession NP_000691

Application

  • Application

    Antibody used in WB on mouse fibroblasts at 1:600.

  • Application

    Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody ａnd Lane B: Primary Antibody + Blocking Peptide ).

  • Application

    Antibody used in IHC on Human Intestine.

  • Application

    Antibody used in WB on Human HepG2 at 1.25 ug/ml.

Positive Control 1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, ａnd HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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