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聯(lián)系我們
聯(lián)系人：楊燕燕
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技術(shù)文章
FoxP1 Catalog Number：OM629218
Product Profile
| Product Name | FoxP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Synthetic peptide within C-terminal of human FoxP1. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-1:2,000: IHC-P:1:200-1:1,000: FC:1:50-1:100: | |
| Species Reactivity | HumanRat |
| Concentration | 1 mg/mL |
Target Information
| Alternative Names | More |
|---|---|
| Molecular Weight(MW) | 75 kDa (Predicted band size) |
| Cellular Localization | Nucleus. |
Database Links
| SwissProt ID | Q9H334 Q498D1 |
|---|
Application
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Application
Fig1: Western blot analysis of FoxP1 on different lysates. Proteins were transferred to a PVDF membrane ａnd blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: rat spleen tissue lysate
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Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin ａnd mounted with DPX.
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Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin ａnd mounted with DPX.
-
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the primary antibody (ER1901-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin ａnd mounted with DPX.
-
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FoxP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the primary antibody (ER1901-26, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin ａnd mounted with DPX.
-
Application
Fig6: Flow cytometric analysis of FoxP1 was done on MCF-7 cells. The cells were fixed, permeabilized ａnd stained with the primary antibody (ER1901-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
| Positive Control | HepG2, rat spleen tissue, rat brain tissue, human colon tissue, human breast tissue, human tonsil tissue, MCF-7. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:200-1:1,000: FC:1:50-1:100: |
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
原創(chuàng)作者：上海篤瑪生物科技有限公司
