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MARK3 [H12-G2] Catalog Number：OM627647

點擊次數(shù)：31 發(fā)布時間：2024/9/5 14:26:00
 

Product Profile

Product Name MARK3 [H12-G2]
Antibody Type Primary Antibodies
Product description 
MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 ａnd is ubiquitously expressed in various human tissue ａnd cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family ａnd the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity ａnd acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 ａnd mouse chromosome 12 F1.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBIHCFC
 
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

HumanMouse
Concentration 2 mg/mL.

Target Information

Alternative Names
C-TAK1 antibody Cdc25C associated protein kinase 1 antibody Cdc25C-associated protein kinase 1 antibody cTAK1 antibody ELKL motif kinase 2 antibody EMK-2 antibody Emk2 antibody ETK 1 antibody KIAA4230 antibody KP78 antibody MAP microtubule affinity regulating kinase 3 antibody MAP/microtubule affinity-regulating kinase 3 antibody Mark3 antibody MARK3_HUMAN antibody Par 1a antibody PAR1A antibody Protein kinase STK10 antibody Ser/Thr protein kinase PAR-1 antibody Serine threonine protein kinase p78 antibody Serine/threonine-protein kinase p78 antibody SerThr protein kinase PAR 1 antibody
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Molecular Weight(MW) 84 kDa
Cellular Localization Cell membrane. Peripheral membrane protein.

Database Links

SwissProt ID P27448

Application

  • Application

    Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution. Positive control：Line1:HeLaLine2:SK-N-SHLine3:K562Line4:HCT116Line5:HEK293Line6:3T3L1Line7:NIH3T3Line8:JurkatLine9:A431

  • Application

    Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.

  • Application

    Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human MARK3 recombinant protein, HeLa, SK-N-SH, K562, HCT116, HEK293, 3T3L1, NIH3T3, Jurkat, ａnd A431 cell lysate, human bladder cancer tissue, SK-N-SH.
Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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