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MARK3 [JE44-84] Catalog Number：OM638410

點擊次數(shù)：29 發(fā)布時間：2024/9/5 14:24:12
 

Product Profile

Product Name MARK3 [JE44-84]
Antibody Type Primary Antibodies
Product description 
MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 ａnd is ubiquitously expressed in various human tissue ａnd cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family ａnd the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity ａnd acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 ａnd mouse chromosome 12 F1.
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Immunogen 
Recombinant protein within human Mark3 aa 600-750.

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBIPICC/IFIHCFC
 
WB:1:500
IP:1:10-1:50
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
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Species Reactivity

HumanMouseRat
Concentration 1 mg/ml.

Target Information

Alternative Names
C-TAK1 antibody Cdc25C associated protein kinase 1 antibody Cdc25C-associated protein kinase 1 antibody cTAK1 antibody ELKL motif kinase 2 antibody EMK-2 antibody Emk2 antibody ETK 1 antibody KIAA4230 antibody KP78 antibody MAP microtubule affinity regulating kinase 3 antibody MAP/microtubule affinity-regulating kinase 3 antibody Mark3 antibody MARK3_HUMAN antibody Par 1a antibody PAR1A antibody Protein kinase STK10 antibody Ser/Thr protein kinase PAR-1 antibody Serine threonine protein kinase p78 antibody Serine/threonine-protein kinase p78 antibody SerThr protein kinase PAR 1 antibody
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Molecular Weight(MW) 84 kDa
Cellular Localization Cell membrane. Peripheral membrane protein.

Database Links

SwissProt ID
P27448
Q03141
Q8VHF0

Application

  • Application

    Fig1: Western blot analysis of MARK3 on different lysates. Proteins were transferred to a PVDF membrane ａnd blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: rat brain tissue lysate Lane 2: A431 cell lysate Lane 3: 293 cell lysate

  • Application

    Fig2: Immunocytochemistry staining MARK3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature ａnd blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Application

    Fig3: Immunocytochemistry staining MARK3 in SH-SY-5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature ａnd blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Application

    Fig4: Immunocytochemistry staining MARK3 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature ａnd blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MARK3 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (ET7109-39) at 1/100 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin ａnd mounted with DPX.

  • Application

    Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MARK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (ET7109-39) at 1/100 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin ａnd mounted with DPX.

  • Application

    Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MARK3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O ａnd PBS, ａnd then probed with the antibody (ET7109-39) at 1/100 dilution, for 30 minutes at room temperature ａnd detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin ａnd mounted with DPX.

  • Application

    Fig8: Flow cytometric analysis of MARK3 was done on MCF-7 cells. The cells were fixed, permeabilized ａnd stained with MARK3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

Positive Control Rat brain tissue lysate, A431, 293, MCF-7, SH-SY-5Y, SiHa, human appendix tissue, human breast cancer tissue, mouse small intestine tissue.
Application Notes
WB:1:500
IP:1:10-1:50
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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