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聯(lián)系人：楊燕燕
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技術(shù)文章
CD55 Antibody (N-term) Catalog Number：OM229252
Product Profile
| Product Name | CD55 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCFC |
| WB~~1:100~500 IF~~1:10~50 IHC~~1:10~50 FC~~1:10~50: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
Target Information
| Gene Synonyms | |
|---|---|
| Alternative Names | More |
| Molecular Weight(MW) | 41400 Da |
| Function | This protein recognizes C4b ａnd C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b ａnd C3b are locally generated during C4 ａnd c3 activation Interaction of daf with cell-associated C4b ａnd C3b polypeptides interferes with their ability to catalyze the conversion of C2 ａnd factor B to enzymatically active C2a ａnd Bb ａnd thereby prevents the formation of C4b2a ａnd C3bBb, the amplification convertases of the complement cascade More |
| Tissue Specificity | Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, ａnd variants of the molecule are present in body fluids ａnd in extracellular matrix |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein |
Database Links
| Entrez Gene | 1604 |
|---|---|
| Nucleotide Accession | NP_000565.1;NP_001108224.1 |
Application
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Application
CD55 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD55 antibody detected the CD55 protein (arrow).
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Application
Confocal immunofluorescent analysis of CD55 Antibody (N-term)(Cat#AP14798a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
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Application
CD55 Antibody (N-term) immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.This data demonstrates the use of CD55 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Application
CD55 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Application Notes | WB~~1:100~500 IF~~1:10~50 IHC~~1:10~50 FC~~1:10~50: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
原創(chuàng)作者：上海篤瑪生物科技有限公司
