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技術(shù)文章

DAF Antibody Catalog Number:OM276710

點(diǎn)擊次數(shù):25 發(fā)布時間:2024/9/5 14:14:52
 

Product Profile

Product Name DAF Antibody
Antibody Type Primary Antibodies
Product description
The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).1) Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44.
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Immunogen
DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF. The immunogen is located within amino acids 360 - 410 of DAF.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAIFIHC-PWB

 
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
Species Reactivity

Human

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol CD55
Alternative Names
Decay-accelerating factor
CD55 molecule decay accelerating factor for complement (Cromer blood group)
CD55
CR
TC
CROM
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Molecular Weight(MW) Predicted: 48 kDa Observed: 49 kDa
Sequence Similarities 2) Cat. No. 10-101 - Human Lung Tissue Slide
Tissue Specificity DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.

Database Links

Entrez Gene 1604
Protein Accession NP_001108224

Application

  • Application

    Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 μg/ml.

  • Application

    Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 μg/ml.

  • Application

    Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 μg/ml.

Positive Control 1) Cat. No. 1203 - A549 Cell Lysate
Application Notes
DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:

Additional Information

Form Liquid
Storage Instructions DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Storage Buffer DAF antibody is supplied in PBS containing 0.02% sodium azide.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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