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技術文章

CD55 Catalog Number：OM628855

點擊次數(shù)：31 發(fā)布時間：2024/9/5 13:49:41
 

Product Profile

Product Name CD55
Antibody Type Primary Antibodies
Product description 
CD55, also called decay accelerating factor (DAF), is a GPI-anchored single chain glycoprotein. CD55 may play a role in protecting cells from complement-mediated lysis by preventing the amplification steps of the complement cascade. CD55 functions to prevent the assembly of C3 convertase or to accelerate the disassembly of preformed convertase, which blocks formation of the membrane attack complex. CD55 is expressed on cells in contact with serum, including hemaoietic ａnd many non-hemaoietic cells.
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Immunogen 
Recombinant protein with Human CD55 aa 150-380.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHC
 
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Species Reactivity

Human
Concentration 1 mg/mL.

Target Information

Alternative Names
CD 55 antibody CD55 antibody CD55 antigen antibody CD55 Cromer blood group system antibody CD55 molecule (Cromer blood group) antibody CD55 molecule antibody CD55 molecule
decay accelerating factor for complement (Cromer blood group) antibody Cd55a antibody Complement decay accelerating factor antibody Complement decay-accelerating factor antibody Complement decay-accelerating factor
GPI-anchored antibody CR antibody CROM antibody Cromer Blood Group antigen antibody Cromer blood group system antibody DAF antibody Daf-GPI antibody DAF_HUMAN antibody Daf1 antibody Dcay accelerating factor for complement (CD55
Cromer blood group system) antibody Decay accelarating factor 1
isoform CRA_a antibody Decay accelerating factor (GPI-form) antibody Decay Accelerating Factor for Complement antibody Decay accelerating factor GPI-form antibody Decay accelerating factor soluble-form antibody GPI-DAF antibody TC antibody
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Molecular Weight(MW) 42/49/60 kDa (Predicted band size)
Cellular Localization Cell membrane. Secreted.

Database Links

SwissProt ID P08174

Application

  • Application

    Fig1: Western blot analysis of CD55 on human plasma using anti-CD55 antibody at 1/1,000

  • Application

    Fig2: Western blot analysis of CD55 on Hela ａnd PC-3M cell lysates using anti-CD55 antibody at 1/500 dilution.

  • Application

    Fig3: ICC staining CD55 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD55 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD55 antibody. Counter stained with hematoxylin.

Positive Control Hela ａnd PC-3M cell lysates, human plasma, human colon cancer tissue, human placenta tissue.
Application Notes
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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