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技術(shù)文章

DKK3 Antibody Catalog Number：OM277084

點擊次數(shù)：26 發(fā)布時間：2024/9/4 11:58:33
 

roduct Profile

Product Name DKK3 Antibody
Antibody Type Primary Antibodies
Product description 
DKK3, like DKK1, DKK2, ａnd DKK4, possesses an N-terminal signal peptide ａnd 2 conserved cysteine-rich domains, which are separated by a linker region ａnd contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, ａnd DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, ａnd spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, ａnd heart.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
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Immunogen 
This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol DKK3
Alternative Names
Dickkopf-related protein 3
Dickkopf-3
Dkk-3
hDkk-3
DKK3
REIC
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Molecular Weight(MW) 38 kDa

Database Links

Entrez Gene 27122
Protein Accession Q9UBP4

Application

  • Application

    Formalin-fixed ａnd paraffin-embedded human breast carcinoma tissue reacted with DKK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Formalin-fixed ａnd paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line ａnd mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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