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技術文章

PDE1B Antibody Catalog Number：OM287663

點擊次數(shù)：32 發(fā)布時間：2024/9/3 14:15:15
 

Product Profile

Product Name PDE1B Antibody
Antibody Type Primary Antibodies
Product description 
Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP ａnd cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
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Immunogen 
This PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-500 amino acids from the C-terminal region of human PDE1B.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB
 
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol PDE1B
Alternative Names
Calcium/calmodulin-dependent 3＇
5＇-cyclic nucleotide phosphodiesterase 1B
Cam-PDE 1B
63 kDa Cam-PDE
PDE1B
PDE1B1
PDES1B
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Molecular Weight(MW) 61 kDa

Database Links

Entrez Gene 5153
Protein Accession Q01064

Application

  • Application

    PDE1B Antibody immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody ａnd DAB staining.

  • Application

    Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Application

    Western blot analysis in mouse brain tissue lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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