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PDE1B [A3-C4] Catalog Number：OM627606

點(diǎn)擊次數(shù)：29 發(fā)布時(shí)間：2024/9/3 14:10:55
 

Product Profile

Product Name PDE1B [A3-C4]
Antibody Type Primary Antibodies
Product description 
PDEs are key enzymes in signaling pathways that influence smooth muscle tone regulation. The PDE1 family are calmodulin-dependent (CaM-PDEs) that undergo stimulation through a calcium-calmodulin complex. Human PDE1B (PDE1B1) protein is present in neuronal cells of the cerebellum, hippocampus, ａnd caudate, ａnd lymphoblastoid lines, such as RPMI-8392 cells. PDE1B may participate in learning, memory, ａnd regulation of phosphorylation of DARPP-32 in dopaminergic neurons. A splice variant known as PDE1B2 encodes a 516-amino acid protein ａnd diverges from PDE1B1 by the replacement of the first 38 residues with an alternative 18 residues. The human PDE1B gene maps to chromosome 12q13, contains 13 exons, ａnd encodes a 536 amino acid protein.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBIHCFC
 
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

HumanRat
Concentration 2 mg/mL.

Target Information

Alternative Names
5＇＇-cyclic nucleotide phosphodiesterase 1B antibody 63 kDa Cam PDE antibody 63 kDa Cam-PDE antibody Calcium/calmodulin dependent 3＇ 5＇ cyclic nucleotide Phosphodiesterase 1B antibody Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody Calcium/calmodulin-dependent 3＇＇ antibody Calmodulin stimulated phosphodiesterase PDE1B1 antibody Cam PDE 1B antibody Cam PDE1B antibody Cam-PDE 1B antibody PDE 1B antibody PDE1B antibody PDE1B_HUMAN antibody PDE1B1 antibody PDES 1B antibody PDES1B antibody Phosphodiesterase 1B antibody Phosphodiesterase 1B calmodulin dependent antibody Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B antibody
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Molecular Weight(MW) 61 kDa
Cellular Localization Cylasm.

Database Links

SwissProt ID Q01064
Q01066

Application

  • Application

    Fig1: Western blot analysis of PDE1B on human PDE1B recombinant protein using anti-PDE1B antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of PDE1B on HEK293 (1) ａnd PDE1B-hIgGFc transfected HEK293 (2) cell lysate using anti-PDE1B antibody at 1/1,000 dilution.

  • Application

    Fig3: Western blot analysis of PDE1B on PC-12 cell lysate using anti-PDE1B antibody at 1/1,000 dilution.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PDE1B antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Flow cytometric analysis of A549 cells with PDE1B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Positive Control Human PDE1B recombinant protein, PDE1B-hIgGFc transfected HEK293 cell lysate, PC-12 cell lysate, human ovarian cancer tissue, human rectum cancer tissue, A549.
Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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