企業(yè)檔案

Product Name	GUCY1A3 [B2-G6]
Antibody Type	Primary Antibodies
Product description	Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble forms, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor forms, known as GC, are peptide hormone receptors. GCS, a cGMP-synthesizing enzyme, is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity ａnd neurotransmission. GCS is a heme containing heterodimer, consisting of one α subunit, designated GCS-α-1, ａnd one β subunit. The heme moeity mediates NO activation, ａnd this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO ａnd CO stimulation are enhanced by the allosteric activator 3-(5＇-hydroxymethyl-2＇furyl)-benzyl -indazole, YC-1. YC-1 can also stimulate GCS in a NO-independent manner. Both the α ａnd β subunits are required for cGMP generation, ａnd at least two isoforms exist for each subunit. Heterodimers consisting of α-1/β-1 ａnd α-2/β-1 have been identified, ａnd both display similar enzymatic activity. More
Immunogen	Recombinant protein

Clonality	Monoclonal
Isotype	IgG1
Host Species	Mouse
Tested Applications	WBICCIHCFC
	WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	Human
Concentration	2 mg/mL.

Alternative Names	GC S alpha 1 antibody GC SA3 antibody GCS alpha 1 antibody GCS alpha 3 antibody GCS-alpha-1 antibody GCS-alpha-3 antibody GCYA3_HUMAN antibody Guanylate cyclase 1 soluble alpha 3 antibody Guanylate cyclase soluble subunit alpha 1 antibody Guanylate cyclase soluble subunit alpha 3 antibody Guanylate cyclase soluble subunit alpha-3 antibody Guanylate cyclase nitric oxide-sensitive alpha-1 subunit antibody Guc1a1 antibody GUC1A3 antibody GUCA3 antibody GUCSA3 antibody Gucy1a1 antibody Gucy1a3 antibody MYMY6 antibody nitric oxide-sensitive Guanylate cyclase alpha-1 subunit antibody NOGC alpha-1 subunit antibody Soluble guanylate cyclase large subunit antibody More
Molecular Weight(MW)	77 kDa
Cellular Localization	Cylasm.

SwissProt ID	Q02108

Application

Fig1: Western blot analysis of GUCY1A3 on HEK293 (1) ａnd GUCY1A3-hIgGFc transfected HEK293 (2) cell lysate using anti-GUCY1A3 antibody at 1/1,000
Application

Fig2: Western blot analysis of GUCY1A3 on different cell lysate using anti-GUCY1A3 antibody at 1/1,000 dilution. Positive control： Line1:HEK293 Line2:Raji
Application

Fig3: ICC staining GUCY1A3 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig4: Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GUCY1A3 antibody. Counter stained with hematoxylin.
Application

Fig6: Flow cytometric analysis of HEK293 cells with GUCY1A3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

Positive Control	GUCY1A3-hIgGFc transfected HEK293 cell lysate, HEK293, Raji cell lysate, HepG2, human renal cancer tissue, human esophageal cancer tissue, HEK293.
Application Notes	WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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