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Product Name	WHSC2 [H8-B11]
Antibody Type	Primary Antibodies
Product description	NELF-A, for negative elongation factor A, is a protein factor required for DRB-sensitive transcription. NELF-A is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) ａnd negative transcription elongation factors, DSIF ａnd NELF. DSIF ａnd NELF act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT. NELF-A is also known as WHSC2 (Wolf-Hirschhorn syndrome candidate 2). Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental ａnd developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3). The human NELF-A gene maps to chromosome 4p16.3 ａnd encodes a 528 amino acid protein that is expressed in endothelial cells. More
Immunogen	Recombinant protein

Clonality	Monoclonal
Isotype	IgG2b
Host Species	Mouse
Tested Applications	WBICCIHCFC
	WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More
Species Reactivity	HumanRat
Concentration	2 mg/mL.

Alternative Names	FLJ10442 antibody FLJ25112 antibody Negative elongation factor A antibody Negative elongation factor polypeptide A antibody NELF A antibody NELF-A antibody NELFA antibody NELFA_HUMAN antibody P/OKcl.15 antibody P/OKcl15 antibody WHSC 2 antibody Whsc2 antibody Wolf Hirschhorn syndrome candidate 2 antibody Wolf Hirschhorn syndrome candidate 2 protein antibody Wolf-Hirschhorn syndrome candidate 2 protein antibody More
Molecular Weight(MW)	57 kDa
Cellular Localization	Nucleus.

SwissProt ID	Q9H3P2 Q5U2N1

Application

Fig1: Western blot analysis of WHSC2 on human WHSC2 recombinant protein using anti-WHSC2 antibody at 1/1,000 dilution.
Application

Fig2: Western blot analysis of WHSC2 on HEK293 (1) ａnd WHSC2-hIgGFc transfected HEK293 (2) cell lysate using anti-WHSC2 antibody at 1/1,000 dilution.
Application

Fig3: Western blot analysis of WHSC2 on different cell lysate using anti-WHSC2 antibody at 1/1,000 dilution. Positive control： Line1:Jurkat Line2:HeLa Line3:HEK293 Line4:Rat brain Line5:A549 Line6:SPC-A-1
Application

Fig4: ICC staining WHSC2 (green) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application

Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
Application

Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-WHSC2 antibody. Counter stained with hematoxylin.
Application

Fig7: Flow cytometric analysis of HEK293 cells with WHSC2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

Positive Control	Human WHSC2 recombinant protein, WHSC2-hIgGFc transfected HEK293 cell lysate, Jurkat, HeLa, HEK293, A549, SPC-A-1 cell lysate, Rat brain tissue lysate, HeLa, human colon cancer tissue, human ovarian cancer tissue, HEK293.
Application Notes	WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. More

Form	Liquid
Storage Instructions	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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