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技術(shù)文章

WHSC2 Catalog Number:OM640691

點(diǎn)擊次數(shù):23 發(fā)布時(shí)間:2024/9/2 14:02:45
 

Product Profile

Product Name WHSC2
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCICCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

Human

Target Information

Alternative Names
NELFA
NELF-A
P/OKcl.15
Molecular Weight(MW) 57.3kDa

Database Links

Entrez Gene 7469

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)

  • Application

    Figure 2: Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.

  • Application

    Figure 3: Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (4), and SPC-A-1 (5) cell lysate.

  • Application

    Figure 4: Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

  • Application

    Figure 5: Flow cytometric analysis of HeLa cells using WHSC2 mouse mAb (green) and negative control (purple).

  • Application

    Figure 6: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.

  • Application

    Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC:1:100~1:200
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Additional Information

Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者:上海篤瑪生物科技有限公司

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