企業(yè)檔案

Gene Synonyms	GRP78
Alternative Names	HSPA5 GRP78 78 kDa glucose-regulated protein Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein More
Molecular Weight(MW)	72333 Da
Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER
Tissue Specificity	This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.
Cellular Localization	Endoplasmic reticulum lumen. Melanosome. Cylasm (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Entrez Gene	3309

Application

The anti-HSPA5 Pab is used in Western blot to detect HSPA5 in mouse liver tissue lysate. HSPA5 (arrow) was detected using the purified Pab.
Application

Western blot analysis of anti-HSPA5 Pab in HL60 cell line lysates (35ug/lane).HSPA5(arrow) was detected using the purified Pab (1:60dilution).
Application

Formalin-fixed ａnd paraffin-embedded human prostata carcinoma tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application

Formalin-fixed ａnd paraffin-embedded human Testis tissue reacted with HSPA5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application

Confocal immunofluorescent analysis of HSPA5 Antibody(Cat#AP1335a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application

HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes	WB~~1:100~500 IHC~~1:50~100 IF~~1:10~50 FC~~1:10~50:

Form	Liquid
Storage Instructions	For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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