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HSPA5 Antibody (C-term) Catalog Number：OM235481

點擊次數(shù)：32 發(fā)布時間：2024/8/30 15:08:04
  

Product Profile

Product Name HSPA5 Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC
 
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
GRP78
Alternative Names
HSPA5
GRP78
78 kDa glucose-regulated protein
Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
Heat shock 70 kDa protein 5
Immunoglobulin heavy chain-binding protein
More 
Molecular Weight(MW) 72333 Da
Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER
Tissue Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.
Cellular Localization Endoplasmic reticulum lumen. Melanosome. Cylasm (By similarity). Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV

Database Links

Entrez Gene 3309
Nucleotide Accession NP_005338

Application

  • Application

    HSPA5 Antibody (C-term) western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).

  • Application

    HSPA5 Antibody (C-term) immunohistochemistry analysis in formalin fixed ａnd paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody ａnd DAB staining. This data demonstrates the use of the HSPA5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • Application

    HSPA5 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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