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技術(shù)文章

GRP78 antibody Catalog Number：OM255573

點(diǎn)擊次數(shù)：30 發(fā)布時間：2024/8/30 15:06:36
  

Product Profile

Product Name GRP78 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to GRP78Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins ａnd degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
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Immunogen 
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Hspa5. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
GRP-78 antibody
heat shock 70kD protein 5 (glucose-regulated protein
78kD) antibody
glucose regulated protein
78 kDa antibody
immunoglobulin heavy chain-binding protein antibody
78 kDa glucose-regulated protein antibody
heat shock 70 kDa protein 5 a
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Molecular Weight(MW) 72 kDa (note) (2)
Tissue Specificity Knockdown/Knockout validation was supported by customer review data.
Cellular Localization Endoplasmic reticulum lumen , Melanosome

Application

  • Application

    Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

  • Application

    Hspa5 antibody detects Hspa5 protein by Western blot analysis. A. 50 µg Rat heart lysate/extract B. 50 µg Rat Lung lysate/extract C. 50 µg Rat Liver lysate/extract D. 50 µg Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000

  • Application

    Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Hspa5 antibody dilution: 1:500.

  • Application

    Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 E: A375 7.5% SDS PAGE diluted at 1:10000

  • Application

    Sample (30 ug of whole cell lysate) A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000

  • Application

    Hspa5 antibody detects Hspa5 protein at cylasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.

  • Application

    Hspa5 antibody detects Hspa5 protein at cylasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.

Positive Control 293T , A431 , HeLa , HepG2 , A375 , NIH-3T-3 , JC , BCL-1 , C2C12 , Raw264.7 , rat heart , rat lung , rat liver , rat kidney
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 0.05% Sodium Azide was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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