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技術(shù)文章

GRP78 antibody Catalog Number：OM255574

點(diǎn)擊次數(shù)：19 發(fā)布時(shí)間：2024/8/30 14:46:30
  

Product Profile

Product Name GRP78 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to GRP78GRP78 (HSPA5), also referred to as ＇immunoglobulin heavy chain-binding protein＇ (BiP), is a member of the heat-shock protein-70 (HSP70) family ａnd is involved in the folding ａnd assembly of proteins in the endoplasmic reticulum (ER). Because so many ER pr
Immunogen 
Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021]

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

FACSICC/IFWB
 
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity

HumanMouseChickenRatSheep
Predicted Reactivity Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%)
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
BIP
FLJ26106
MIF2
GRP78
More 
Target HSPA5
Tissue Specificity Grp78
Cellular Localization Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Application

  • Application

    Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

  • Application

    Detection of Bip/Grp78 on HeLa whole cell extracts using

Application Notes
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:

Additional Information

Form Liquid
Storage Instructions Store at 4C short term. Aliquot ａnd store at -20C long term. Avoid freeze-thaw cycles.
Storage Buffer PBS, 30% glycerol ａnd 0.1% sodium azide was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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