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技術(shù)文章

Grp78 antibody Catalog Number:OM255578

點(diǎn)擊次數(shù):18 發(fā)布時(shí)間:2024/8/30 14:43:42
  

Product Profile

Product Name Grp78 antibody
Antibody Type Primary Antibodies
Product description
Mouse Monoclonal antibody [9E4-2A7-H6] to Grp78The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
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Immunogen
Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 9E4-2A7-H6
Host Species Mouse
Tested Applications

ICC/IFIHC-PWB

 
ICC/IF:1:200~1:500
IHC-P:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

HumanRat

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
GRP78
BIP
HSPA5
heat shock 70kDa protein 5 (glucose-regulated protein
78kDa)
MIF2
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Target Grp78

Application

  • Application

    WB analysis of various cell lysates using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:1000.

  • Application

    ICC/IF analysis of HeLa cells using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:50.

  • Application

    IHC-P analysis of colorectal cancer using Grp78 antibody [9E4-2A7-H6] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Application Notes
ICC/IF:1:200~1:500
IHC-P:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Additional Information

Form Liquid
Storage Instructions Stored at -20°C
Storage Buffer PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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