企業(yè)檔案

Product Name	Grp78 antibody , C-term
Antibody Type	Primary Antibodies
Product description	Rabbit Polyclonal antibody to Grp78 (78 kDa glucose-regulated protein)When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as ＇immunoglobulin heavy chain-binding protein＇ (BiP), is a member of the heat-shock protein-70 (HSP70) family ａnd is involved in the folding ａnd assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM] More
Immunogen	Recombinant protein encompassing a sequence within the C-terminus region of human Grp78. The exact sequence is proprietary.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WB
	*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity	HumanMouse
Predicted Reactivity	Rat, Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (98%), Zebrafish (88%), Japanese medaka (87%), Xenopus laevis (95%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%)
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	BIP antibody FLJ26106 antibody GRP78 antibody MIF2 antibody HSPA5 antibody immunoglobulin heavy chain-binding protein antibody 78 kDa glucose-regulated protein antibody endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody "heat shock More
Molecular Weight(MW)	72 kDa (note)
Cellular Localization	Endoplasmic reticulum lumen , Melanosome

Application

Non-transfected (–) ａnd transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with Grp78 antibody [C2C3], C-term diluted at 1:1000.
Application

Grp78 antibody detects Grp78 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Grp78 antibody dilution: 1:1000
Application

Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:2000

Positive Control	HepG2 , NIH-3T3 , JC , BCL-1
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher. :

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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