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技術文章

CD33 Catalog Number:OM640668

點擊次數(shù):24 發(fā)布時間:2024/8/29 14:36:21
 

Product Profile

Product Name CD33
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human CD33 (AA: 15-237) expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

Human

Target Information

Alternative Names
p67
SIGLEC3
SIGLEC-3
Molecular Weight(MW) 39.8kDa

Database Links

Entrez Gene 945

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)

  • Application

    Figure 2: Western blot analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.

  • Application

    Figure 3: Western blot analysis using CD33 mouse mAb against THP-1 (1) cell lysate.

  • Application

    Figure 5: Flow cytometric analysis of HeLa cells using CD33 mouse mAb (green) and negative control (purple).

  • Application

    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.

  • Application

    Figure 7: Immunohistochemical analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Ascitic fluid containing 0.03% sodium azide.
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者:上海篤瑪生物科技有限公司

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