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技術文章

CD33 (SIGLEC3) Antibody (N-term)

點擊次數(shù)：28 發(fā)布時間：2024/8/29 14:33:29
 

Product Profile

Product Name CD33 (SIGLEC3) Antibody (N-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHC
 
WB~~1:100~500 IHC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
SIGLEC3
Alternative Names
CD33
SIGLEC3
Myeloid cell surface antigen CD33
Sialic acid-binding Ig-like lectin 3
gp67
CD_antigen=CD33
Flags: Precursor
More 
Molecular Weight(MW) 39825 Da
Function
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cylasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro)
More 
Tissue Specificity Monocytic/myeloid lineage cells.
Cellular Localization Cell membrane; Single-pass type I membrane protein

Database Links

Entrez Gene 945

Application

  • Application

    The anti-Siglec3 N-term Pab is used in Western blot to detect Siglec3 in jurkat cell lysate.

  • Application

    Formalin-fixed ａnd paraffin-embedded human lymph with CD33 (SIGLEC3) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Application Notes
WB~~1:100~500 IHC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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