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CD33 [B7-C6] Catalog Number：OM627666

點(diǎn)擊次數(shù)：27 發(fā)布時(shí)間：2024/8/29 14:27:43
 

Product Profile

Product Name CD33 [B7-C6]
Antibody Type Primary Antibodies
Product description 
CD33 is a type I transmembrane glycoprotein that is found on granulocyte ａnd macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.
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Immunogen 
Recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

WBIHCFC
 
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Species Reactivity

Human
Concentration 2 mg/mL.

Target Information

Alternative Names
CD 33 antibody CD33 antibody CD33 antigen (gp67) antibody CD33 antigen antibody CD33 molecule antibody CD33_HUMAN antibody FLJ00391 antibody gp67 antibody My9 antibody Myeloid cell surface antigen CD33 antibody Myeloid cell surface antigen CD33 precursor antibody Myeloid differentiation antigen CD33 antibody p67 antibody Sialic acid binding Ig like lectin 3 antibody Sialic acid binding immunoglobulin like lectin 3 antibody Sialic acid-binding Ig-like lectin 3 antibody SIGLEC 3 antibody Siglec-3 antibody SIGLEC3 antibody
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Molecular Weight(MW) 70 kDa
Cellular Localization Cell membrane.

Database Links

SwissProt ID P20138

Application

  • Application

    Fig1: Western blot analysis of CD33 on human CD33 recombinant protein using anti-CD33 antibody at 1/1,000 dilution.

  • Application

    Fig2: Western blot analysis of CD33 on HEK293 (1) ａnd CD33-hIgGFc transfected HEK293 (2) cell lysate using anti-CD33 antibody at 1/1,000 dilution.

  • Application

    Fig3: Western blot analysis of CD33 on THP-1 (1)  cell lysate using anti-CD33 antibody at 1/1,000 dilution.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CD33 antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CD33 antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Flow cytometric analysis of Hela cells with CD33 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

Positive Control Human CD33 recombinant protein, CD33-hIgGFc transfected HEK293 cell lysate, THP-1 cell lysate, human esophageal cancer tissue, human endometrium tissue, Hela.
Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
FC:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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