企業(yè)檔案

Product Name	CD33
Antibody Type	Primary Antibodies
Product description	Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cylasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). More
Immunogen	KLH conjugated synthetic peptide derived from mouse CD33 101-200/334

Alternative Names	CD33_HUMAN CD 33 CD33 antigen CD33 molecule FLJ00391 gp67 Myeloid cell surface antigen CD33 Myeloid cell surface antigen CD33 precursor p67 Sialic acid binding Ig like lectin 3 Sialic acid binding immunoglobulin like lectin 3 SIGLEC3 Siglec-3 myeloid cell surface antigen CD33 isoform 1 precursor. More
Molecular Weight(MW)	40/37 KDa
Cellular Localization	Cell membrane; Single-pass type I membrane protein.

SwissProt ID	Q63994

Application

Fig1: Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti- CD33 antibody(ER1905-89), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1905-89, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application

Fig2: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-CD33 (ER1905-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/37 kD Observed band size: 40 kD

Positive Control	Monocytic/myeloid lineage cells.
Application Notes	WB:1:500-2000: FC:1μg/Test:

Form	Liquid
Storage Instructions	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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