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技術文章

CD33 Antibody Catalog Number：OM274456

點擊次數(shù)：21 發(fā)布時間：2024/8/29 14:21:12
 

Product Profile

Product Name CD33 Antibody
Antibody Type Primary Antibodies
Product description 
CD33 Antibody: CD33 is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family that is highly expressed on myeloid progenitor cells. Assessment of CD33 expression is of great importance in the immunodiagnosis of acute leukemia, allowing distinction between myeloid ａnd lymphoid origin, as CD33 is generally restricted to the myelomonocytic lineage. CD33 can associate with the protein-tyrosine phosphatases SHP-1 ａnd SHP-2 ａnd thus could modulate downstream signaling events associated with cell activation. Common variants of CD33 have been found to be associated with late-onset Alzheimer＇s disease.1) Freeman SD, Kelm S, Barber EK, et al. Characterization of CD33 as a new member of the sialoadhesion family of cellular interaction molecules. Blood 1995; 85:2005-12.
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Immunogen 
CD33 antibody was raised against a 14 amino acid synthetic peptide near the center of human CD33. The immunogen is located within amino acids 180 - 230 of CD33.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAWB
 
CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol CD33
Alternative Names
CD33 Antibody: p67
SIGLEC3
SIGLEC-3
Myeloid cell surface antigen CD33
Sialic acid-binding Ig-like lectin 3
Siglec-3
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Tissue Specificity At least three isoforms of CD33 are known to exist; this antibody will detect all three isoforms.

Database Links

Entrez Gene 945
Protein Accession NP_001763

Application

  • Application

    Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 ａnd (B) 2 μg/mL.

Positive Control 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
CD33 antibody can be used for detection of CD33 by Western blot at 1 - 2 μg/mL.:

Additional Information

Form Liquid
Storage Instructions CD33 antibody can be stored at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer CD33 Antibody is supplied in PBS containing 0.02% sodium azide.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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