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聯(lián)系人：楊燕燕
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技術(shù)文章
Phospho-GATA6-Y271 Catalog Number：OM217336
Product Profile
| Product Name | Phospho-GATA6-Y271 |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFDB |
| IF~~1:100 DB~~1:100~500: | |
| Species Reactivity | Human |
| Concentration | 1 mg/ml |
| Purification |
Target Information
| Alternative Names | |
|---|---|
| Molecular Weight(MW) | 60033 Da |
| Function | Transcriptional activator that regulates SEMA3C ａnd PLXNA2. Thought to be important for regulating terminal differentiation and/or proliferation |
| Tissue Specificity | Expressed in heart, gut ａnd gut-derived tissues |
| Cellular Localization | Nucleus. |
Database Links
| Entrez Gene | 2627 |
|---|---|
| Nucleotide Accession | NP_005248.2 |
Application
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Application
Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3766a phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.
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Application
Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
| Application Notes | IF~~1:100 DB~~1:100~500: |
|---|
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
原創(chuàng)作者：上海篤瑪生物科技有限公司
