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技術文章

FGF4 Antibody Catalog Number：OM278802

點擊次數(shù)：32 發(fā)布時間：2024/8/26 14:32:02
  

Product Profile

Product Name FGF4 Antibody
Antibody Type Primary Antibodies
Product description 
FGF4 Antibody: Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic ａnd cell survival activities ａnd play key roles in growth ａnd survival of stem cells during embryogenesis, tissue regeneration, ａnd carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity ａnd is a potent angiogenic factor, expressed in several highly vascularized tumors ａnd also in adult mouse testis, intestine, ａnd brain. Studies on the mouse homolog suggests a function in bone morphogenesis ａnd limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation ａnd neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells ａnd a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.1) Powers CJ, McLeskey SW, ａnd Wellstein A. Fibroblast growth factors, their receptors ａnd signaling. Endocr. Relat. Cancer2000; 7:165-97.
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Immunogen 
FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICCIFWB
 
FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.:
Species Reactivity

HumanMouse
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol FGF4
Alternative Names
FGF4 Antibody: HST
KFGF
HST-1
HSTF1
K-FGF
HBGF-4
HST
KS3
Fibroblast growth factor 4
Heparin secretory-transforming protein 1
FGF-4
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Sequence Similarities 2) Cat. No. 17-201 - 3T3/BALB Cell Slide

Database Links

Entrez Gene 2249
Protein Accession P08620

Application

  • Application

    Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.

  • Application

    Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.

  • Application

    Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 ａnd (B) 1 μg/mL.

Positive Control 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.:

Additional Information

Form Liquid
Storage Instructions FGF4 antibody can be stored at 4˚C for three months ａnd -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer FGF4 Antibody is supplied in PBS containing 0.02% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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