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技術(shù)文章

FLT3 antibody Catalog Number：OM253326

點擊次數(shù)：18 發(fā)布時間：2024/8/26 13:43:33
 

Product Profile

Product Name FLT3 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to FLT3FLT3 encodes a class III receptor tyrosine kinase that regulates hemaoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, ａnd a cylasmic kinase domain split into two parts by a kinase-ｉnsert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to auhosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates ａnd activates multiple cylasmic effector molecules in pathways involved in apoptosis, proliferation, ａnd differentiation of hemaoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia ａnd acute lymphoblastic leukemia.
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Immunogen 
Peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ICC/IFWB
 
Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
CD135
FLK2
STK1
FLT3
FLT3 receptor tyrosine kinase
STK-1
FLT-3
CD135 antigen
fetal liver kinase-2
stem cell tyrosine kinase 1
tyrosine-protein kinase receptor FLT3
growth factor receptor tyrosine kinase type III
fms-like tyrosine kinase 3
FL c
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Target FLT3
Molecular Weight(MW) 130, 160 (note)

Application

  • Application

    Western blot analysis of extracts from 293 ａnd MCF cells using FLT3 Antibody .

  • Application

    Immunofluorescence staining of methanol-fixed Hela cells using FLT3 Antibody .

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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