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Product Name	BRCA1 antibody - ChIP grade
Antibody Type	Primary Antibodies
Product description	Mouse monoclonal [17F8] to BRCA1BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, ａnd does not recognize the Aexon 11 splice variant. In a high proportion of breast ａnd ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cylasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation. More
Immunogen	Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Clonality	Monoclonal
Isotype	IgG1
Clone Number	17F8
Host Species	Mouse
Tested Applications	ELISAICC/IFIHCIHC-PIPWBChIP
	For ICC/IF: Use at a concentration of 1 µg/ml. For WB: 1:500-1:3000. For IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher:
Species Reactivity	HumanMouse
Concentration	1 mg/ml
Purification	Protein G

Gene Synonyms	113705 RNF53 BRCA1 BRCAI 672 PSCP BRCC1 P38398 IRIS BROVCA1 PNCA4 More
Target	BRCA1
Molecular Weight(MW)	220 kDa (note)
Tissue Specificity	This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast ａnd ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cylasm.
Cellular Localization	Nuclear/Cylasmic
Light Chain Type	kappa

Application

BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, ａnd blotted with BRCA1 antibody [17F8] - ChIP grade diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application

BRCA1 antibody 6B4 ａnd BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 ａnd 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
Application

BRCA1 antibody 6B4 ａnd BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), ａnd mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
Application

Non-transfected (–) ａnd transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, ａnd the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Positive Control	293T , A431 , HeLa
Application Notes	For ICC/IF: Use at a concentration of 1 µg/ml. For WB: 1:500-1:3000. For IHC-P: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher:

Form	Liquid
Storage Instructions	Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot ａnd store at -20°C or below. Avoid freeze-thaw cycles.
Storage Buffer	Phosphate-buffered saline, pH7.2 containing no preservatives

原創(chuàng)作者：上海篤瑪生物科技有限公司

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