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技術文章

BRCA1 antibody Catalog Number：OM241915

點擊次數(shù)：22 發(fā)布時間：2024/8/23 13:22:27
 

Product Profile

Product Name BRCA1 antibody
Antibody Type Primary Antibodies
Product description 
Mouse monoclonal [9F12] to BRCA1BRCA1-9F12 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast ａnd ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cylasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
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Immunogen 
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Key Feature

Clonality Monoclonal
Isotype IgG1
Clone Number 9F12
Host Species Mouse
Tested Applications

ICC/IFIPWB
 
For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1-10 µg/ml. Predicated molecular weight: 208 kDa. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Protein G

Target Information

Gene Synonyms
113705
RNF53
BRCA1
BRCAI
672
PSCP
BRCC1
P38398
IRIS
BROVCA1
PNCA4
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Target BRCA1
Molecular Weight(MW) 220 kDa (note)
Cellular Localization Nuclear
Light Chain Type kappa

Application

  • Application

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRCA1 antibody at 1:100 dilution.

Positive Control Any normal human tissue, especially those containing rapidly proliferating cells such as lymphoid germinal centers
Application Notes
For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1-10 µg/ml. Predicated molecular weight: 208 kDa. Optimal dilutions/concentrations should be determined by the researcher.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20°C or below. Avoid freeze-thaw cycles
Storage Buffer Phosphate-buffered saline, pH7.2, no preservative added

原創(chuàng)作者：上海篤瑪生物科技有限公司

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