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GCLM antibody Catalog Number：OM254278

點擊次數(shù)：36 發(fā)布時間：2024/8/22 13:46:02
 

Product Profile

Product Name GCLM antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to GCLM (glutamate-cysteine ligase, modifier subunit)Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit ａnd a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]
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Immunogen 
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFIHC-PWB
 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human
Predicted Reactivity Mouse, Rat, BovineMouse (100%), Rat (100%), Bovine (100%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
GLCLR antibody
GCLM antibody
GSC light chain antibody
gamma-ECS regulatory subunit antibody
GCS light chain antibody
"glutamate-cysteine ligase (gamma-glutamylcysteine synthetase)
regulatory (30.8kD) antibody"
glutamate－－cysteine ligase modifier sub
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Molecular Weight(MW) 31 kDa (note) (2)

Application

  • Application

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GCLM antibody at 1:200 dilution.

  • Application

    Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using GCLM antibody at 1:500 dilution.

  • Application

    Non-transfected (–) ａnd transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody [N1N3] diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control HeLa , 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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