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技術(shù)文章

GSC Catalog Number:OM640464

點(diǎn)擊次數(shù):24 發(fā)布時間:2024/8/22 13:31:21
 

Product Profile

Product Name GSC
Antibody Type Primary Antibodies
Immunogen
Purified recombinant fragment of human GSC (AA: 191-257) expressed in E. Coli.

Key Feature

Clonality Monoclonal
Host Species Unknow
Tested Applications

WBIHCFCMICC/IF

 
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 
Species Reactivity

Human

Target Information

Alternative Names
GSC
Molecular Weight(MW) 28.2kDa

Database Links

Entrez Gene 145258

Application

  • Application

    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

  • Application

    Figure 1: Western blot analysis using GSC mAb against human GSC recombinant protein. (Expected MW is 33.5 kDa)

  • Application

    Figure 2: Western blot analysis using GSC mAb against HEK293 (1) and GSC (AA: 191-257)-hIgGFc transfected HEK293 (2) cell lysate.

  • Application

    Figure 4: Flow cytometric analysis of Hela cells using GSC mouse mAb (green) and negative control (red).

  • Application

    Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using GSC mouse mAb with DAB staining.

  • Application

    Figure 6: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GSC mouse mAb with DAB staining.

Application Notes
WB:1:250~1:1000
IHC:1:20~1:100
ICC/IF:1:100~1:200
Notes:Optimal dilutions/concentrations should be determined by the researcher.
More 

Additional Information

Form Purified antibody in PBS with 0.05% sodium azide
Storage Instructions 4℃; -20℃ for long term storage

原創(chuàng)作者:上海篤瑪生物科技有限公司

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