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技術(shù)文章

PI3K p85/p55 (phospho Tyr467/199) antibody Catalog Number：OM268505

點(diǎn)擊次數(shù)：27 發(fā)布時(shí)間：2024/8/22 11:29:48
 

Product Profile

Product Name PI3K p85/p55 (phospho Tyr467/199) antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to PI3K P85 (Phospho Tyr467)Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, ａnd acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake ａnd glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain ａnd regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, ａnd acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
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Immunogen 
Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3K P85.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

WB
 
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
GRB1
p85
p85-ALPHA
PIK3R1
phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
phosphatidylinositol 3-kinase
regulatory subunit
polypeptide 1 (p85 alpha)
PI3K regulatory subunit alpha
ptdIns-3-kinase regulatory subunit alpha
phosphatidyl
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Target PI3K P85 (Phospho Tyr467)
Molecular Weight(MW) 85 (note)

Application

  • Application

    Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) Antibody .

Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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