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聯(lián)系人:楊燕燕
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技術(shù)文章
Anti-PI3K p85 alpha + Gamma antibody Catalog Number:OM642782
Product Profile
Product Name | Anti-PI3K p85 alpha + Gamma antibody |
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Antibody Type | Primary Antibodies |
Immunogen | Polypeptide |
Key Feature
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | ELISAICC/IFIHCWB |
WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:50-1:200 | |
Species Reactivity | HumanMouseRat |
Concentration | 1mg/ml |
Purification | Protein A |
Target Information
Gene Symbol | PIK3R1 |
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Gene Synonyms | More |
Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] More |
Molecular Weight(MW) | 83 kDa(Observed:50 kDa) |
Cellular Localization | Cylasmic |
Application
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WB
Figure 1: Western blot analysis using PI3K p85 alpha + Gamma antibody against Nih3t3(1) cell lysate.
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IHC
Figure 2: Immunohistochemical analysis of paraffin-em
bedded breast cancer tissues using PI3K p85 alpha + Gamma antibody with DAB staining. -
ICC/IF
Figure 3: Immunofluorescence analysis of A549 cells using PI3K p85 alpha + Gamma antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:50-1:200 |
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Additional Information
Form | Liquid |
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Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
原創(chuàng)作者:上海篤瑪生物科技有限公司