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技術(shù)文章

Anti-PI3K p85 alpha + Gamma antibody Catalog Number:OM642782

點(diǎn)擊次數(shù):48 發(fā)布時(shí)間:2024/8/21 14:03:42
  

Product Profile

Product Name Anti-PI3K p85 alpha + Gamma antibody
Antibody Type Primary Antibodies
Immunogen
Polypeptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICC/IFIHCWB

 
WB:1:500-1:2000
IHC:1:200-1:1000
ICC:1:50-1:200
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Protein A

Target Information

Gene Symbol PIK3R1
Gene Synonyms
p85
AGM7
GRB1
IMD36
p85alpha
p85-ALPHA
More 
Gene Full Name phosphoinositide-3-kinase regulatory subunit 1
Gene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
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Molecular Weight(MW) 83 kDa(Observed:50 kDa)
Cellular Localization Cylasmic

Application

  • WB

    Figure 1: Western blot analysis using PI3K p85 alpha + Gamma antibody against Nih3t3(1) cell lysate.

  • IHC

    Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PI3K p85 alpha + Gamma antibody with DAB staining.

  • ICC/IF

    Figure 3: Immunofluorescence analysis of A549 cells using PI3K p85 alpha + Gamma antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes
WB:1:500-1:2000
IHC:1:200-1:1000
ICC:1:50-1:200

Additional Information

Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% PC300.
 

原創(chuàng)作者:上海篤瑪生物科技有限公司

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