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技術(shù)文章

TLR4 Antibody (H-80) Catalog Number：OM181327

點擊次數(shù)：24 發(fā)布時間：2024/8/21 13:44:04
 

Product Profile

Product Name TLR4 Antibody (H-80)
Antibody Type Primary Antibodies
Modification Notes Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities ａnd designated toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo ａnd also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cylasmic Toll/IL-1R-like domain. TLR2 ａnd TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation ａnd translocation of NFkB ａnd suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macro-phages, ａnd monocytes. TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAIFIHCIPWB
 
WB:1:100~1:200
IP:1:20~1:100
IF:1:20~1:100
IHC:1:10~1:50
More 
Species Reactivity

HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epie corresponding to amino acids 242-321 mapping to an internal region of TLR4 (Toll-like receptor 4) of human origin
Tissue Specificity epie corresponding to amino acids 242-321 mapping to an internal region of TLR4 (Toll-like receptor 4) of human origin

Database Links

Entrez Gene 7099

Application

  • Application

    Western blot analysis of TLR4 expression in THP-1 whole cell lysate.

  • Application

    Western blot analysis of TLR4 expression in HEL 92.1.7 (A) ａnd HL-60 (B) whole cell lysates.

Application Notes
WB:1:100~1:200
IP:1:20~1:100
IF:1:20~1:100
IHC:1:10~1:50
More 

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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