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技術(shù)文章

CD31 Antibody Catalog Number：OM274439

點(diǎn)擊次數(shù)：33 發(fā)布時間：2024/8/20 14:27:38
  

Product Profile

Product Name CD31 Antibody
Antibody Type Primary Antibodies
Product description 
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein ａnd a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, ａnd concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells ａnd platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 ａnd CD34 can be used as markers for myeloid progenitor cells ａnd recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).1) Mayr U et al. Circ Res 98:412-20 (2006).
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Immunogen 
Ni-NTA purified recombinant CD31 expressed in E. Coli strain BL21 (DE3).

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications

ELISAIHCWB
 
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Symbol PECAM1
Alternative Names
EndoCAM
PECAM-1
CD31
PECA1
GPIIA＇
endoCAM
CD31/EndoCAM
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Database Links

Entrez Gene 5175
Protein Accession P16284

Application

  • Application

    Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.

  • Application

    Immunohistochemical analysis of paraffin - embedded human lymphonodus tissue showing cylasmic location of vascular endothelial cells using CD31 antibody with DAB staining.

Application Notes
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:

Additional Information

Form Liquid
Storage Instructions CD31 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Ascitic fluid containing 0.03% sodium azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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