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技術(shù)文章

KEAP1 antibody Catalog Number:OM260526

點(diǎn)擊次數(shù):15 發(fā)布時(shí)間:2024/8/19 14:02:46

Product Profile

Product Name KEAP1 antibody
Antibody Type Primary Antibodies
Product description
Rabbit polyclonal antibody to KEAP1 (kelch-like ECH-associated protein 1)This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cylasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]
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Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human KEAP1. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ICC/IFWB

 
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity

Human

Predicted Reactivity Mouse, Rat, Pig, Chimpanzee, BovineMouse (96%), Rat (96%), Pig (99%), Chimpanzee (100%), Bovine (98%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
INrf2 antibody
KIAA0132 antibody
KLHL19 antibody
MGC10630 antibody
MGC1114 antibody
MGC20887 antibody
MGC4407 antibody
MGC9454 antibody
KEAP1 antibody
cytosolic inhibitor of Nrf2 antibody
kelch-like ECH-associated protein 1 antibody
kelch-like p
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Target KEAP1
Molecular Weight(MW) 70 kDa (note) (2)
Cellular Localization Cylasm , Nucleus

Application

  • Application

    Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000

  • Application

    KEAP1 antibody [C1C3] detects KEAP1 protein at cylasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Positive Control NT2D1 , IMR32 , MCF-7
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher. :

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
 

原創(chuàng)作者:上海篤瑪生物科技有限公司

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