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技術(shù)文章

NFE2L2 Antibody (N-term) Catalog Number：OM215273

點擊次數(shù)：21 發(fā)布時間：2024/8/19 14:01:20
 

Product Profile

Product Name NFE2L2 Antibody (N-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB
 
WB~~1:100~500:
Species Reactivity

Human
Concentration 1 mg/ml
Purification  

Target Information

Gene Synonyms
NRF2
Alternative Names
NFE2L2
NRF2
Nuclear factor erythroid 2-related factor 2
HEBP1
Nuclear factor
erythroid derived 2
like 2
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Molecular Weight(MW) 67827 Da
Function
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region
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Tissue Specificity Widely expressed. Highest expression in adult muscle, kidney, lung, liver ａnd in fetal muscle
Cellular Localization Cylasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents

Database Links

Entrez Gene 4780
Nucleotide Accession NP_006155

Application

  • Application

    Western blot analysis of anti-NFE2L2 Antibody (N-term) in Ramos cell line lysates (35ug/lane). Nrf2(arrow) was detected using the purified Pab (1:60 dilution).

  • Application

    Western blot analysis of Nrf2(Nfe2l2)(arrow) using rabbit polyclonal Nrf2(Nfe2l2) Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Nrf2(Nfe2l2) gene (Lane 2) (Origene Technologies).

Application Notes
WB~~1:100~500:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high ａnd low pH buffers ａnd neutralized immediately, followed by dialysis against PBS.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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