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技術文章

NRF2 antibody , Internal

點擊次數：14 發(fā)布時間：2024/8/19 13:46:30
 

Product Profile

Product Name NRF2 antibody , Internal
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)NFE2 (MIM 601490), NFE2L1 (MIM 163260), ａnd NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) ａnd FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ChIPICC/IFIHC-PIPWB
 
WB:1:200~1:1000
IP:1:50~1:200
ICC/IF:1:50~1:250
IHC-p:1:25~1:100
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Species Reactivity

HumanMouseRat
Predicted Reactivity Rhesus Monkey, BovineRhesus Monkey (99%), Bovine (84%)
Concentration 0.5 mg/ml
Purification Affinity purified

Target Information

Gene Synonyms
NRF2 antibody
NFE2L2 antibody
nuclear factor erythroid-derived 2-like 2 antibody
nuclear factor
erythroid derived 2
like 2 antibody
NF-E2-related factor 2 antibody
HEBP1 antibody
nuclear factor erythroid 2-related factor 2 antibody
NFE2-related fa
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Molecular Weight(MW) 68 kDa (note) (8)
Cellular Localization Cylasm , cytosol , Nucleus

Application

  • Application

    NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.

  • Application

    Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal . Western blot analysis was performed using NRF2 antibody [N2C2], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

  • Application

    ChIP was performed with HepG2 chromatin extract ａnd 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

  • Application

    Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2 antibody at 1:100 dilution.

  • Application

    NRF2 antibody [N2C2], Internal detects NRF2 protein at cylasm ａnd nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

  • Application

    NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) ａnd NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Whole cell extract (30 µg) was separated by 5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Untreated (–) ａnd treated (+) MDA-MB-231 nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T , C8D30
Application Notes
WB:1:200~1:1000
IP:1:50~1:200
ICC/IF:1:50~1:250
IHC-p:1:25~1:100
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Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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