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技術(shù)文章

Occludin Antibody (H-279) Catalog Number:OM200043

點(diǎn)擊次數(shù):30 發(fā)布時間:2024/8/16 13:52:28
  

Product Profile

Product Name Occludin Antibody (H-279)
Antibody Type Primary Antibodies
Modification Notes Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAIFIPWB

 
WB:1:100~1:200
IP:1:20~1:100
IF:1:20~1:100
Species Reactivity

HumanMouseRat

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Alternative Names
epie corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin
Tissue Specificity epie corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin

Database Links

Entrez Gene 4950

Application

  • Application

    Western blot analysis of Occludin expression in HeLa (A), COLO 320DM (B), ECV304 (C), MDCK (D), T84 (E) and A-431 (F) whole cell lysates.

  • Application

    Western blot analysis of Occludin isoform expression in MDCK (A), COLO 320DM (B) and ECV304 (C) whole cell lysates.

Application Notes
WB:1:100~1:200
IP:1:20~1:100
IF:1:20~1:100

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

原創(chuàng)作者:上海篤瑪生物科技有限公司

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