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聯(lián)系人:楊燕燕
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技術(shù)文章
Occludin Antibody (H-279) Catalog Number:OM200043
Product Profile
Product Name | Occludin Antibody (H-279) |
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Antibody Type | Primary Antibodies |
Modification Notes | Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction. |
Key Feature
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | ELISAIFIPWB |
WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 | |
Species Reactivity | HumanMouseRat |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Target Information
Alternative Names | |
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Tissue Specificity | epie corresponding to amino acids 132-411 mapping within an internal region of Occludin of human origin |
Database Links
Entrez Gene | 4950 |
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Application
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Application
Western blot analysis of Occludin ex
pression in HeLa (A), COLO 320DM (B), ECV304 (C), MDCK (D), T84 (E) and A-431 (F) whole cell lysates. -
Application
Western blot analysis of Occludin isoform ex
pression in MDCK (A), COLO 320DM (B) and ECV304 (C) whole cell lysates.
Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
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Additional Information
Form | Liquid |
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Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
原創(chuàng)作者:上海篤瑪生物科技有限公司