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技術(shù)文章

Occludin antibody Catalog Number：OM266243

點(diǎn)擊次數(shù)：29 發(fā)布時(shí)間：2024/8/16 13:51:58
  

Product Profile

Product Name Occludin antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to OccludinThis gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation ａnd maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq]
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Immunogen 
Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICC/IFIHC-FIHC-PWB
 
WB:1:500~1:2000
IHC-p:1:50~1:200
IHC-f:1:100~1:500
*Optimal dilutions/concentrations should be determined by the researcher.:
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Species Reactivity

HumanMonkeyMouseRat
Predicted Reactivity Mouse, Dog, RatMouse (89%), Dog (89%), Rat (87%)
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
BLCPMG antibody
OCLN antibody
occludin antibody
tight junction protein occludin TM4 minus antibody
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Molecular Weight(MW) 59 kDa (note) (3)
Cellular Localization Membrane; Multi-pass membrane protein , Cell junction , tight junction

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin antibody at 1:200 dilution.

  • Application

    Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

  • Application

    Occludin antibody detects Occludin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Occludin protein stained by Occludin antibody diluted at 1:250. Blue: Fluoroshield with DAPI .

Positive Control HepG2
Application Notes
WB:1:500~1:2000
IHC-p:1:50~1:200
IHC-f:1:100~1:500
*Optimal dilutions/concentrations should be determined by the researcher.:
Hide 

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
 

原創(chuàng)作者：上海篤瑪生物科技有限公司

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