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Occludin [JJ091-08] Catalog Number：OM637700

點擊次數(shù)：21 發(fā)布時間：2024/8/16 11:47:56
 

Product Profile

Product Name Occludin [JJ091-08]
Antibody Type Primary Antibodies
Product description 
Occludin is an integral membrane protein closely associated with the tight junctions of epithelial ａnd endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial ａnd endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization ａnd the function of occludin. Expression of occludin is up-regulated by poly-unsaturated fatty acids, increasing transendothelial cell resistance ａnd reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression ａnd discontinuous distribution. Up-regulation of epithelial occludin may play a role in enhancing paracellular permeability ａnd be related to the damage to the tight junction.
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Immunogen 
recombinant protein

Key Feature

Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications

WBIPFC
 
WB:1:1,000
FC:1:50-1:100
Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Tight junction protein occludin antibody
Molecular Weight(MW) 59 kDa
Cellular Localization Cell membrane, Cell junction.

Database Links

SwissProt ID Q16625
Q61146
Q6P6T5

Application

  • Application

    Fig1: Flow cytometric analysis of 293 cells with Occludin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Positive Control 293
Application Notes
WB:1:1,000
FC:1:50-1:100

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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