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技術文章

Caspase 9 antibody Catalog Number：OM243367

點擊次數(shù)：27 發(fā)布時間：2024/8/15 13:46:12
  

Product Profile

Product Name Caspase 9 antibody
Antibody Type Primary Antibodies
Product description 
Rabbit Polyclonal antibody to Caspase 9Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves ａnd activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
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Immunogen 
Peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

WB
 
Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity

Human
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified

Target Information

Gene Synonyms
APAF-3
APAF3
CASPASE-9c
ICE-LAP6
MCH6
CASP9
apoptotic protease MCH-6
caspase-9
apoptotic protease activating factor 3
protein phosphatase 1
regulatory subunit 56
ICE-like apoptotic protease 6
caspase 9
apoptosis-related cysteine peptidase
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Target Caspase 9
Molecular Weight(MW) 47, 37, 35 (note)
Tissue Specificity It could both recognize pro-form caspase 9 ａnd caspase 9 subunit p35.

Application

  • Application

    Western blot analysis of extracts from 293 ａnd Hela cells using Caspase 9 Antibody .

Application Notes
Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:

Additional Information

Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer saline (without Mg2+ ａnd Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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