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技術(shù)文章

Occludin

點(diǎn)擊次數(shù)：27 發(fā)布時(shí)間：2024/8/14 15:44:59
  

Product Profile

Product Name Occludin
Antibody Type Primary Antibodies
Product description 
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration ａnd polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
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Immunogen 
Synthetic peptide within human Occludin aa 1-50 conjugated to Keyhole Limpet Haemocyanin (KLH).

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBICCIHCFC
 
WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
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Species Reactivity

HumanMouseRat
Concentration 1 mg/mL.

Target Information

Alternative Names
BLCPMG antibody FLJ08163 antibody FLJ18079 antibody FLJ77961 antibody FLJ94056 antibody MGC34277 antibody Occludin antibody Ocln antibody OCLN_HUMAN antibody Phosphatase 1 regulatory subunit 115 antibody PPP1R115 antibody PTORCH1 antibody Tight junction protein occludin antibody
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Molecular Weight(MW)

59 kDa
Cellular Localization

Cell membrane.

Application

  • Application

    Fig1: Western blot analysis of Occludin on mouse kidney (1) ａnd human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.

  • Application

    Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Application

    Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.

  • Application

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.

  • Application

    Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Positive Control

mouse kidney ａnd human kidney tissue lysates, HepG2, Hela, A549, human lung tissue, mouse lung tissue.
Application Notes
WB:1:500-2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
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Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

原創(chuàng)作者：上海篤瑪生物科技有限公司

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